GLYCOSIDE HYDROLASE FAMILY 16 - Avhandlingar.se

Keep solution chilled on ice during assay. Catalase Solution – • Catalase products supplied as a lyophilized powder. a. Prepare an initial solution of 10 mg/ml in cold Phosphate Buffer (this solution will be slightly hazy). b. Cyclodextrin glycosyltransferase (CGTase) is an enzyme able to convert starch and other substrates into cyclodextrins (CDs).

Cgtase enzyme assay

( Masataka et al., 1995). transferase (EC 2.4.1.9) is a unique enzyme capable media composition for better enzyme production CGTase assay: CGTase activity was determined. Synonyms. cyclodextrin glycosyltransferase, cyclodextrin glucanotransferase, cyclomaltodextrin glucanotransferase, alpha-cgtase, beta-cgtase, toruzyme, 10 May 2013 glycosyltransferase (CGTase), produced by bacteria of the genera.

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Cyclodextrin glycosyltransferase, CGTase (E.C. 2.4.1.19) is a hydrolytic enzyme used in the starch hydrolysis for the synthesis of cyclodextrins (CDs). Bacterial CGTase often involved in the production of three major types of CDs, namely α-CD, β-CD and γ-CD at different ratio. γ-Cyclodextrin glycosyltransferase (γ-CGTase) catalyzes the biotransformation of low-cost starch into valuable γ-cyclodextrin (γ-CD), which is widely applied in biotechnology, food, and pharmaceutical industries.

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[9]. One unit of alkaline phosphatase activity is de-¢ned as that In this case, the purity of the enzyme is important, as well as the assay procedure.On SDS-PAGE we observed three bands displaying amylolytic activity, and with IEF we observed four bands, suggesting the presence of possible degradation products of the CGTase. 2013-7-15 · CGTase assay in different temperatures, ranging from 400.C - 900C for 10 min. Then the reaction was done according to the CGTase assay.

The product specificity of γ-CGTase was investigated by high-performance liquid chromatography (HPLC) analysis of three CDs (α-, β-, γ-CD) in the biotransformation product of cassava starch.
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As with the FP assay (Fig. 4B), conversion of FRET signal to cGAMP formation yielded a linear response (data not Alternate assay methods are described by Dinovo et al. (1973). Method: Creatine kinase activity is determined in a coupled enzyme system utilizing pyruvate kinase (PK) and lactate dehydrogenase (LDH).

Therefore, the activity assay is typically carried out with 10-50 mM H2O2. reproducibility of the assay.
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GLYCOSIDE HYDROLASE FAMILY 16 - Avhandlingar.se

Assay of enzyme activityThe cyclization activity of CGTase was measured according to the method established by Kaneko et al. with slight modiWcation.

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(FCCVIII) Invertase Units SU: One Sumner Unit is the quantity of enzyme which will convert 1mg of sucrose to glucose and fructose in 5 minutes under the conditions of the assay (pH 4.5 and 20°C). After 45 days, the preparation of alpha-CGTase still had 100% of the enzyme activity with different additives superimposed at the optimum concentration at 40 degrees C. The CD spectra of alpha-CGTase showed that glycerin could protect the secondary and the tertiary structure of the CGTase under high temperature and therefore the enzyme maintained its high activity. ASSAY PROCEDURE: 1 Pre-equilibrate 0.5 mL aliquots of suitably diluted enzyme in sodium acetate buffer (25 mM, pH 4.5) at 40°C for 5 min. 2 Initiate the reaction by adding a Beta-Glucazyme tablet.

GLYCOSIDE HYDROLASE FAMILY 16 - Avhandlingar.se

The immobilized CGTase maintained 2016-7-20 · for the periplasmic enzyme but without the heat treatment step19. CGTase enzyme assay—The activity of CGTase, determined as dextrinisation activity, was monitored at 60°C for 10 min20. One unit of activity was defined as the amount of enzyme which decreases 10% of amylose-iodine complex optical density per min 2005-2-16 · Enzyme assay CGTase activity was assayed by the Lejeune et al. (1989) method slightly modified by Gawande and Patkar (1999).

CGTase wird in der Stärkeindustrie zur Herstellung von Cyclodextrinen () eingesetzt. Assay of CGTase was carried out according to the method of Kaneko et al., 1987 [2]. The method is described under Analytical Methods.